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This article is cited by 39 publications. Analysis of CPA synthase genes in crude eDNA suggests the presence of additional TD gene clusters in soil environments. The complete synthetic refactoring of a representative gene cluster from the latter eDNA specific group led to the characterization of the erdasporines, cytotoxins with a novel carboxy-indolocarbazole TD substructure. Four of these groups contain clusters from both cultured and culture independent studies, while the remaining group consists entirely of eDNA-derived clusters. Twelve unprecedented TD biosynthetic gene clusters that can be arranged into five groups (A–E) based on their ability to generate distinct TD core substructures were recovered from eDNA libraries. Functional and bioinformatics analyses of TD clusters indicate that CPA synthase gene sequences diverge in concert with the functional output of their respective clusters, making this gene a powerful tool for guiding the discovery of novel TDs from the environment. Here we investigate bacterial tryptophan dimer (TD) biosynthesis by probing environmental DNA (eDNA) libraries for chromopyrrolic acid (CPA) synthase genes.
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